Generated:Article Title: TaqMan PCR for Detection of Vibrio cholerae O1, O139, Non-O1, and Non-O139 in Pure Cultures, Raw Oysters, and Synthetic Seawater
Article Snippet: .. Vibrio strains evaluated and RQ values generated in PCR with TaqMan primers designed to detection V. cholerae strains Bacteria evaluated No. of isolates tested Collection or isolation sourcea RQ valueb Interpretationc Bacteria evaluated No. of isolates tested Collection or isolation sourcea RO valueb Interpretationc Vibrio alginolyticus 5 WL104, L, O 1.0 Negative 85-2-4, L, CF 1.2 Negative WL118, CF, L 0.0 Negative WL121 0.8 Negative WL210, L, O 0.9 Negative Vibrio anguillarum 5 WL83, L, S 0.1 Negative WL87, O 0.2 Negative WL85, L, O 0.0 Negative WL89, L, CF 0.4 Negative 99-053B 0.0 Negative Vibrio cholerae serotype O1 13 ATCC 14035, I 30.1 Positive ATCC 14735, C, I 33.2 Positive 0395N1 34.0 Positive WL11, C, P 35.4 Positive WL13, C 34.0 Positive WL26, S, P 35.2 Positive WL31, C, I 32.2 Positive WL34 33.0 Positive WL36, C, M 35.8 Positive WL41 32.4 Positive WL65 34.4 Positive WL69 33.8 Positive WL99 31.4 Positive Vibrio cholerae serotype O139 13 ATCC 51394 32.1 Positive ATCC 25874, C 30.1 Positive WL29, C, I 33.1 Positive WL32 33.2 Positive WL33 32.0 Positive WL34 35.4 Positive WL42, L, O 34.4 Positive WL50 35.2 Positive WL37 32.2 Positive WL38 33.0 Positive WL39 35.5 Positive WL40 36.8 Positive NCTC 8457, I, C 35.8 Positive a ATCC, American Type Culture Collection; CDC, Centers for Disease Control and Prevention, Atlanta, Ga.; NCTC, National Collection of Type Cultures, London, England. ..
Polymerase Chain Reaction:Article Title: TaqMan PCR for Detection of Vibrio cholerae O1, O139, Non-O1, and Non-O139 in Pure Cultures, Raw Oysters, and Synthetic Seawater
Article Snippet: .. Vibrio strains evaluated and RQ values generated in PCR with TaqMan primers designed to detection V. cholerae strains Bacteria evaluated No. of isolates tested Collection or isolation sourcea RQ valueb Interpretationc Bacteria evaluated No. of isolates tested Collection or isolation sourcea RO valueb Interpretationc Vibrio alginolyticus 5 WL104, L, O 1.0 Negative 85-2-4, L, CF 1.2 Negative WL118, CF, L 0.0 Negative WL121 0.8 Negative WL210, L, O 0.9 Negative Vibrio anguillarum 5 WL83, L, S 0.1 Negative WL87, O 0.2 Negative WL85, L, O 0.0 Negative WL89, L, CF 0.4 Negative 99-053B 0.0 Negative Vibrio cholerae serotype O1 13 ATCC 14035, I 30.1 Positive ATCC 14735, C, I 33.2 Positive 0395N1 34.0 Positive WL11, C, P 35.4 Positive WL13, C 34.0 Positive WL26, S, P 35.2 Positive WL31, C, I 32.2 Positive WL34 33.0 Positive WL36, C, M 35.8 Positive WL41 32.4 Positive WL65 34.4 Positive WL69 33.8 Positive WL99 31.4 Positive Vibrio cholerae serotype O139 13 ATCC 51394 32.1 Positive ATCC 25874, C 30.1 Positive WL29, C, I 33.1 Positive WL32 33.2 Positive WL33 32.0 Positive WL34 35.4 Positive WL42, L, O 34.4 Positive WL50 35.2 Positive WL37 32.2 Positive WL38 33.0 Positive WL39 35.5 Positive WL40 36.8 Positive NCTC 8457, I, C 35.8 Positive a ATCC, American Type Culture Collection; CDC, Centers for Disease Control and Prevention, Atlanta, Ga.; NCTC, National Collection of Type Cultures, London, England. ..
Bacteria:Article Title: TaqMan PCR for Detection of Vibrio cholerae O1, O139, Non-O1, and Non-O139 in Pure Cultures, Raw Oysters, and Synthetic Seawater
Article Snippet: .. Vibrio strains evaluated and RQ values generated in PCR with TaqMan primers designed to detection V. cholerae strains Bacteria evaluated No. of isolates tested Collection or isolation sourcea RQ valueb Interpretationc Bacteria evaluated No. of isolates tested Collection or isolation sourcea RO valueb Interpretationc Vibrio alginolyticus 5 WL104, L, O 1.0 Negative 85-2-4, L, CF 1.2 Negative WL118, CF, L 0.0 Negative WL121 0.8 Negative WL210, L, O 0.9 Negative Vibrio anguillarum 5 WL83, L, S 0.1 Negative WL87, O 0.2 Negative WL85, L, O 0.0 Negative WL89, L, CF 0.4 Negative 99-053B 0.0 Negative Vibrio cholerae serotype O1 13 ATCC 14035, I 30.1 Positive ATCC 14735, C, I 33.2 Positive 0395N1 34.0 Positive WL11, C, P 35.4 Positive WL13, C 34.0 Positive WL26, S, P 35.2 Positive WL31, C, I 32.2 Positive WL34 33.0 Positive WL36, C, M 35.8 Positive WL41 32.4 Positive WL65 34.4 Positive WL69 33.8 Positive WL99 31.4 Positive Vibrio cholerae serotype O139 13 ATCC 51394 32.1 Positive ATCC 25874, C 30.1 Positive WL29, C, I 33.1 Positive WL32 33.2 Positive WL33 32.0 Positive WL34 35.4 Positive WL42, L, O 34.4 Positive WL50 35.2 Positive WL37 32.2 Positive WL38 33.0 Positive WL39 35.5 Positive WL40 36.8 Positive NCTC 8457, I, C 35.8 Positive a ATCC, American Type Culture Collection; CDC, Centers for Disease Control and Prevention, Atlanta, Ga.; NCTC, National Collection of Type Cultures, London, England. ..
Article Title: TaqMan PCR for Detection of Vibrio cholerae O1, O139, Non-O1, and Non-O139 in Pure Cultures, Raw Oysters, and Synthetic Seawater
Article Snippet: .. A complete list of bacterial strains that were evaluated is given in Tables and . table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Bacteria evaluated No. of isolates tested Collection or isolation source a ΔRQ value b Interpretation c Vibrio alginolyticus 5 WL104, L, O 1.0 Negative 85-2-4, L, CF 1.2 Negative WL118, CF, L 0.0 Negative WL121 0.8 Negative WL210, L, O 0.9 Negative Vibrio anguillarum 5 WL83, L, S 0.1 Negative WL87, O 0.2 Negative WL85, L, O 0.0 Negative WL89, L, CF 0.4 Negative 99-053B 0.0 Negative Vibrio cholerae serotype O1 13 ATCC 14035, I 30.1 Positive ATCC 14735, C, I 33.2 Positive 0395N1 34.0 Positive WL11, C, P 35.4 Positive WL13, C 34.0 Positive WL26, S, P 35.2 Positive WL31, C, I 32.2 Positive WL34 33.0 Positive WL36, C, M 35.8 Positive WL41 32.4 Positive WL65 34.4 Positive WL69 33.8 Positive WL99 31.4 Positive Vibrio cholerae serotype O139 13 ATCC 51394 32.1 Positive ATCC 25874, C 30.1 Positive WL29, C, I 33.1 Positive WL32 33.2 Positive WL33 32.0 Positive WL34 35.4 Positive WL42, L, O 34.4 Positive WL50 35.2 Positive WL37 32.2 Positive WL38 33.0 Positive WL39 35.5 Positive WL40 36.8 Positive NCTC 8457, I, C 35.8 Positive Vibrio cholerae serotype non-O1 9 ATCC 25872, C 35.0 Positive ATCC 25873, C 34.0 Positive N2030 35.4 Positive WL102, L, EN 36.0 Positive WL105, L, O 35.2 Positive WL111 32.2 Positive WL115 35.0 Positive WL116 34.4 Positive WL119 35.2 Positive Vibrio cholerae serotype non-O139 3 WL42, L, O 32.2 Positive NCTC 8457, C, I 33.0 Positive WL29, C, I 34.0 Positive Vibrio damsela 3 ATCC 33539, CF, CA 0.0 Negative CDC 23–81, S, F 0.0 Negative CDC 183-79, C, H 0.0 Negative Vibrio fluvialis 2 ATCC 33809, C, I 0.0 Negative CDC 1280-78, C, I 0.0 Negative Vibrio mimicus 2 ATCC 33653,C, NC 2.0 Negative CDC 269–80, S, LA 1.8 Negative Vibrio parahaemolyticus 6 ATCC 17802, C, J 0.0 Negative ATCC 27519, S, LA 0.0 Negative WL78, L, C 0.1 Negative 90-299 0.2 Negative WL90, L, S 0.0 Negative ATCC 49398, C, M 0.3 Negative Vibrio vulnificus 6 ATCC 27562, C 0.0 Negative CDC A1402, C 0.1 Negative 89–142 0.1 Negative WL43, L, O 0.0 Negative CDC A8694, C, F 0.0 Negative WL76, L, S 0.2 Negative Open in a separate window a ATCC, American Type Culture Collection; CDC, Centers for Disease Control and Prevention, Atlanta, Ga.; NCTC, National Collection of Type Cultures, London, England. ..
Isolation:Article Title: TaqMan PCR for Detection of Vibrio cholerae O1, O139, Non-O1, and Non-O139 in Pure Cultures, Raw Oysters, and Synthetic Seawater
Article Snippet: .. Vibrio strains evaluated and RQ values generated in PCR with TaqMan primers designed to detection V. cholerae strains Bacteria evaluated No. of isolates tested Collection or isolation sourcea RQ valueb Interpretationc Bacteria evaluated No. of isolates tested Collection or isolation sourcea RO valueb Interpretationc Vibrio alginolyticus 5 WL104, L, O 1.0 Negative 85-2-4, L, CF 1.2 Negative WL118, CF, L 0.0 Negative WL121 0.8 Negative WL210, L, O 0.9 Negative Vibrio anguillarum 5 WL83, L, S 0.1 Negative WL87, O 0.2 Negative WL85, L, O 0.0 Negative WL89, L, CF 0.4 Negative 99-053B 0.0 Negative Vibrio cholerae serotype O1 13 ATCC 14035, I 30.1 Positive ATCC 14735, C, I 33.2 Positive 0395N1 34.0 Positive WL11, C, P 35.4 Positive WL13, C 34.0 Positive WL26, S, P 35.2 Positive WL31, C, I 32.2 Positive WL34 33.0 Positive WL36, C, M 35.8 Positive WL41 32.4 Positive WL65 34.4 Positive WL69 33.8 Positive WL99 31.4 Positive Vibrio cholerae serotype O139 13 ATCC 51394 32.1 Positive ATCC 25874, C 30.1 Positive WL29, C, I 33.1 Positive WL32 33.2 Positive WL33 32.0 Positive WL34 35.4 Positive WL42, L, O 34.4 Positive WL50 35.2 Positive WL37 32.2 Positive WL38 33.0 Positive WL39 35.5 Positive WL40 36.8 Positive NCTC 8457, I, C 35.8 Positive a ATCC, American Type Culture Collection; CDC, Centers for Disease Control and Prevention, Atlanta, Ga.; NCTC, National Collection of Type Cultures, London, England. ..
Article Title: TaqMan PCR for Detection of Vibrio cholerae O1, O139, Non-O1, and Non-O139 in Pure Cultures, Raw Oysters, and Synthetic Seawater
Article Snippet: .. A complete list of bacterial strains that were evaluated is given in Tables and . table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Bacteria evaluated No. of isolates tested Collection or isolation source a ΔRQ value b Interpretation c Vibrio alginolyticus 5 WL104, L, O 1.0 Negative 85-2-4, L, CF 1.2 Negative WL118, CF, L 0.0 Negative WL121 0.8 Negative WL210, L, O 0.9 Negative Vibrio anguillarum 5 WL83, L, S 0.1 Negative WL87, O 0.2 Negative WL85, L, O 0.0 Negative WL89, L, CF 0.4 Negative 99-053B 0.0 Negative Vibrio cholerae serotype O1 13 ATCC 14035, I 30.1 Positive ATCC 14735, C, I 33.2 Positive 0395N1 34.0 Positive WL11, C, P 35.4 Positive WL13, C 34.0 Positive WL26, S, P 35.2 Positive WL31, C, I 32.2 Positive WL34 33.0 Positive WL36, C, M 35.8 Positive WL41 32.4 Positive WL65 34.4 Positive WL69 33.8 Positive WL99 31.4 Positive Vibrio cholerae serotype O139 13 ATCC 51394 32.1 Positive ATCC 25874, C 30.1 Positive WL29, C, I 33.1 Positive WL32 33.2 Positive WL33 32.0 Positive WL34 35.4 Positive WL42, L, O 34.4 Positive WL50 35.2 Positive WL37 32.2 Positive WL38 33.0 Positive WL39 35.5 Positive WL40 36.8 Positive NCTC 8457, I, C 35.8 Positive Vibrio cholerae serotype non-O1 9 ATCC 25872, C 35.0 Positive ATCC 25873, C 34.0 Positive N2030 35.4 Positive WL102, L, EN 36.0 Positive WL105, L, O 35.2 Positive WL111 32.2 Positive WL115 35.0 Positive WL116 34.4 Positive WL119 35.2 Positive Vibrio cholerae serotype non-O139 3 WL42, L, O 32.2 Positive NCTC 8457, C, I 33.0 Positive WL29, C, I 34.0 Positive Vibrio damsela 3 ATCC 33539, CF, CA 0.0 Negative CDC 23–81, S, F 0.0 Negative CDC 183-79, C, H 0.0 Negative Vibrio fluvialis 2 ATCC 33809, C, I 0.0 Negative CDC 1280-78, C, I 0.0 Negative Vibrio mimicus 2 ATCC 33653,C, NC 2.0 Negative CDC 269–80, S, LA 1.8 Negative Vibrio parahaemolyticus 6 ATCC 17802, C, J 0.0 Negative ATCC 27519, S, LA 0.0 Negative WL78, L, C 0.1 Negative 90-299 0.2 Negative WL90, L, S 0.0 Negative ATCC 49398, C, M 0.3 Negative Vibrio vulnificus 6 ATCC 27562, C 0.0 Negative CDC A1402, C 0.1 Negative 89–142 0.1 Negative WL43, L, O 0.0 Negative CDC A8694, C, F 0.0 Negative WL76, L, S 0.2 Negative Open in a separate window a ATCC, American Type Culture Collection; CDC, Centers for Disease Control and Prevention, Atlanta, Ga.; NCTC, National Collection of Type Cultures, London, England. ..
Control:Article Title: TaqMan PCR for Detection of Vibrio cholerae O1, O139, Non-O1, and Non-O139 in Pure Cultures, Raw Oysters, and Synthetic Seawater
Article Snippet: .. Vibrio strains evaluated and RQ values generated in PCR with TaqMan primers designed to detection V. cholerae strains Bacteria evaluated No. of isolates tested Collection or isolation sourcea RQ valueb Interpretationc Bacteria evaluated No. of isolates tested Collection or isolation sourcea RO valueb Interpretationc Vibrio alginolyticus 5 WL104, L, O 1.0 Negative 85-2-4, L, CF 1.2 Negative WL118, CF, L 0.0 Negative WL121 0.8 Negative WL210, L, O 0.9 Negative Vibrio anguillarum 5 WL83, L, S 0.1 Negative WL87, O 0.2 Negative WL85, L, O 0.0 Negative WL89, L, CF 0.4 Negative 99-053B 0.0 Negative Vibrio cholerae serotype O1 13 ATCC 14035, I 30.1 Positive ATCC 14735, C, I 33.2 Positive 0395N1 34.0 Positive WL11, C, P 35.4 Positive WL13, C 34.0 Positive WL26, S, P 35.2 Positive WL31, C, I 32.2 Positive WL34 33.0 Positive WL36, C, M 35.8 Positive WL41 32.4 Positive WL65 34.4 Positive WL69 33.8 Positive WL99 31.4 Positive Vibrio cholerae serotype O139 13 ATCC 51394 32.1 Positive ATCC 25874, C 30.1 Positive WL29, C, I 33.1 Positive WL32 33.2 Positive WL33 32.0 Positive WL34 35.4 Positive WL42, L, O 34.4 Positive WL50 35.2 Positive WL37 32.2 Positive WL38 33.0 Positive WL39 35.5 Positive WL40 36.8 Positive NCTC 8457, I, C 35.8 Positive a ATCC, American Type Culture Collection; CDC, Centers for Disease Control and Prevention, Atlanta, Ga.; NCTC, National Collection of Type Cultures, London, England. ..
Article Title: TaqMan PCR for Detection of Vibrio cholerae O1, O139, Non-O1, and Non-O139 in Pure Cultures, Raw Oysters, and Synthetic Seawater
Article Snippet: .. A complete list of bacterial strains that were evaluated is given in Tables and . table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Bacteria evaluated No. of isolates tested Collection or isolation source a ΔRQ value b Interpretation c Vibrio alginolyticus 5 WL104, L, O 1.0 Negative 85-2-4, L, CF 1.2 Negative WL118, CF, L 0.0 Negative WL121 0.8 Negative WL210, L, O 0.9 Negative Vibrio anguillarum 5 WL83, L, S 0.1 Negative WL87, O 0.2 Negative WL85, L, O 0.0 Negative WL89, L, CF 0.4 Negative 99-053B 0.0 Negative Vibrio cholerae serotype O1 13 ATCC 14035, I 30.1 Positive ATCC 14735, C, I 33.2 Positive 0395N1 34.0 Positive WL11, C, P 35.4 Positive WL13, C 34.0 Positive WL26, S, P 35.2 Positive WL31, C, I 32.2 Positive WL34 33.0 Positive WL36, C, M 35.8 Positive WL41 32.4 Positive WL65 34.4 Positive WL69 33.8 Positive WL99 31.4 Positive Vibrio cholerae serotype O139 13 ATCC 51394 32.1 Positive ATCC 25874, C 30.1 Positive WL29, C, I 33.1 Positive WL32 33.2 Positive WL33 32.0 Positive WL34 35.4 Positive WL42, L, O 34.4 Positive WL50 35.2 Positive WL37 32.2 Positive WL38 33.0 Positive WL39 35.5 Positive WL40 36.8 Positive NCTC 8457, I, C 35.8 Positive Vibrio cholerae serotype non-O1 9 ATCC 25872, C 35.0 Positive ATCC 25873, C 34.0 Positive N2030 35.4 Positive WL102, L, EN 36.0 Positive WL105, L, O 35.2 Positive WL111 32.2 Positive WL115 35.0 Positive WL116 34.4 Positive WL119 35.2 Positive Vibrio cholerae serotype non-O139 3 WL42, L, O 32.2 Positive NCTC 8457, C, I 33.0 Positive WL29, C, I 34.0 Positive Vibrio damsela 3 ATCC 33539, CF, CA 0.0 Negative CDC 23–81, S, F 0.0 Negative CDC 183-79, C, H 0.0 Negative Vibrio fluvialis 2 ATCC 33809, C, I 0.0 Negative CDC 1280-78, C, I 0.0 Negative Vibrio mimicus 2 ATCC 33653,C, NC 2.0 Negative CDC 269–80, S, LA 1.8 Negative Vibrio parahaemolyticus 6 ATCC 17802, C, J 0.0 Negative ATCC 27519, S, LA 0.0 Negative WL78, L, C 0.1 Negative 90-299 0.2 Negative WL90, L, S 0.0 Negative ATCC 49398, C, M 0.3 Negative Vibrio vulnificus 6 ATCC 27562, C 0.0 Negative CDC A1402, C 0.1 Negative 89–142 0.1 Negative WL43, L, O 0.0 Negative CDC A8694, C, F 0.0 Negative WL76, L, S 0.2 Negative Open in a separate window a ATCC, American Type Culture Collection; CDC, Centers for Disease Control and Prevention, Atlanta, Ga.; NCTC, National Collection of Type Cultures, London, England. ..
other:Article Title: TaqMan PCR for Detection of Vibrio cholerae O1, O139, Non-O1, and Non-O139 in Pure Cultures, Raw Oysters, and Synthetic Seawater
Article Snippet: , ATCC 14735, C, I , 33.2 , Positive.
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